There is renewed interest in the use of nitrate to treat the profundal zone of lakes to inhibit anaerobic biogeochemical processes that result in the degradation of bottom water quality (e.g., sediment phosphorus release, mercury methylation). In this study we used experimental sediment–water interface chambers to quantify the rate of sediment nitrate uptake (SNU) in profundal sediments from Lake Perris, a eutrophic raw water reservoir in Southern California. Deoxygenated chamber water was spiked with nitrate, and nitrate concentration was monitored over time under quiescent conditions, followed by mixed conditions with average water velocities of 1 cm/s. Key findings included: (1) SNU decreased with decreasing nitrate concentration, (2) SNU was higher under mixed versus quiescent conditions by nearly 50%, and (3) nitrate uptake as a function of nitrate concentration followed a conventional sediment oxygen demand model in which nitrate uptake was proportional to the square root of nitrate concentration. The probable mechanism for elevated SNU under mixed conditions was an increased diffusional concentration gradient combined with a decrease in the diffusional boundary layer at the sediment–water interface, both of which enhanced the flux of nitrate from overlaying water into sediment. Managers planning to implement lake nitrate addition should account for induced nitrate demand when determining dosing rates. For example, based on our modeling efforts from this data set, SNU in Lake Perris could range by an order of magnitude, from around 12 mg N/m2 /d under quiescent, low nitrate conditions (0.1 mg N/l) to around 120 mg N/m2 /d under mixed, high nitrate conditions (5 mg N/l).