Publications

2011

Kallosh R, Linde A, Rube T. General inflaton potentials in supergravity. Physical Review D. 2011.

We describe a way to construct supergravity models with an arbitrary inflaton potential V(\phi) and show that all other scalar fields in this class of models can be stabilized at the inflationary trajectory by a proper choice of the Kähler potential.

Behbahani SR, Jankowiak M, Rube T, Wacker JG. Nearly supersymmetric dark atoms. Advances in High Energy Physics. 2011;2011.

Publisher's Version

Theories of dark matter that support bound states are an intriguing possibility for the identity of the missing mass of the Universe. This article proposes a class of models of supersymmetric composite dark matter where the interactions with the Standard Model communicate supersymmetry breaking to the dark sector. In these models, supersymmetry breaking can be treated as a perturbation on the spectrum of bound states. Using a general formalism, the spectrum with leading supersymmetry effects is computed without specifying the details of the binding dynamics. The interactions of the composite states with the Standard Model are computed, and several benchmark models are described. General features of nonrelativistic supersymmetric bound states are emphasized.

2009

Kallosh R, Lee CH, Rube T. N= 8 Supergravity 4-point amplitudes. Journal of High Energy Physics. 2009;2009(02):050.

Publisher's Version

We present the explicit expressions in Script N= 8 supergravity for the bosonic 4-particle tree and 1-loop amplitudes including vectors and scalars. We also present the candidate 4-point UV divergences in a form of helicity amplitudes, corresponding to 3-loop manifestly Script N= 8 supersymmetric and Lorentz covariant counterterm. This may shed some light on the 3-loop finiteness of Script N= 8 SG and on a conjectured higher loop finiteness. We perform a supersymmetric deformation to complex momentum of the 4-point generating function including higher-loop counterterms and the 1-loop UV finite amplitudes. Using the explicit form of the scalar part of the 3-loop counterterm and of the 1-loop UV finite scalar 4-point amplitudes we find that they both have an unbroken E (7, 7) symmetry. We derive from E (7, 7) symmetry the low-energy theorem for the 1-loop n-point amplitudes.

Recent Publications

Gagoski D, Rube T, Rastogi C, Melo LA, Li X, Voleti R, Shah NH, Bussemaker HJ. Accurate sequence-to-affinity models for SH2 domains from multi-round peptide binding assays coupled with free-energy regression. bioRxiv. Forthcoming Forthcoming.
Zhang L, Li G, Zhang Y, Cheng Y, Roberts N, Glenn S, DeZwaan-McCabe D, Rube H, Manthey J, Coleman G, et al. Boosting genome editing efficiency in human cells and plants with novel LbCas12a variants.. Europe PMC. 2023.

Publisher's Version
Feng S, Rastogi C, Loker RE, Glassford WJ, Rube T, Bussemaker HJ, Mann RS. Transcription factor paralogs orchestrate alternative gene regulatory networks by context-dependent cooperation with multiple cofactors. bioRxiv. 2022.

Publisher's Version

In eukaryotes, members of large transcription factor families often exhibit similar DNA binding properties in vitro, yet initiate paralog-specific gene regulatory networks in vivo. The serially homologous first (T1) and third (T3) thoracic legs of Drosophila, which result from alternative gene regulatory networks specified by the Hox proteins Scr and Ubx, respectively, offer a unique opportunity to address this paradox in vivo. Genome-wide analyses using epitope-tagged alleles of both Hox loci in the T1 and T3 leg imaginal discs, which are the precursors to the adult appendages and ventral body regions, show that ∼8% of Hox binding is paralog-specific. Binding specificity is mediated by interactions with distinct cofactors in different domains: the known Hox cofactor Exd acts in the proximal domain and is necessary for Scr to bind many of its paralog-specific targets, while in the distal leg domain, we identified the homeodomain protein Distal-less (Dll) as a novel Hox cofactor that enhances Scr binding to a different subset of genomic loci. Reporter genes confirm the in vivo roles of Scr+Dll and suggest that ∼1/3 of paralog-specific Hox binding in enhancers is functional. Together, these findings provide a genome-wide view of how Hox paralogs, and perhaps paralogs of other transcription factor families, orchestrate alternative downstream gene networks and suggest the importance of multiple, context-specific cofactors.
Rube T, Rastogi C, Feng S, Kribelbauer JF, Li A, Becerra B, Melo LA, Do BV, Li X, Adam HH, et al. Prediction of protein-ligand binding affinity from sequencing data with interpretable machine learning. Nat. Biotechnol. 2022.

Publisher's Version

Protein-ligand interactions are increasingly profiled at high throughput using affinity selection and massively parallel sequencing. However, these assays do not provide the biophysical parameters that most rigorously quantify molecular interactions. Here we describe a flexible machine learning method, called ProBound, that accurately defines sequence recognition in terms of equilibrium binding constants or kinetic rates. This is achieved using a multi-layered maximum-likelihood framework that models both the molecular interactions and the data generation process. We show that ProBound quantifies transcription factor (TF) behavior with models that predict binding affinity over a range exceeding that of previous resources; captures the impact of DNA modifications and conformational flexibility of multi-TF complexes; and infers specificity directly from in vivo data such as ChIP-seq without peak calling. When coupled with an assay called KD-seq, it determines the absolute affinity of protein-ligand interactions. We also apply ProBound to profile the kinetics of kinase-substrate interactions. ProBound opens new avenues for decoding biological networks and rationally engineering protein-ligand interactions.
Basu A, Bobrovnikov DG, Qureshi Z, Kayikcioglu T, Ngo TTM, Ranjan A, Eustermann S, Cieza B, Morgan MT, Hejna M, et al. Measuring DNA mechanics on the genome scale. Nature. 2021;589(7842):462–467.

Publisher's Version

Mechanical deformations of DNA such as bending are ubiquitous and have been implicated in diverse cellular functions1. However, the lack of high-throughput tools to measure the mechanical properties of DNA has limited our understanding of how DNA mechanics influence chromatin transactions across the genome. Here we develop 'loop-seq'-a high-throughput assay to measure the propensity for DNA looping-and determine the intrinsic cyclizabilities of 270,806 50-base-pair DNA fragments that span Saccharomyces cerevisiae chromosome V, other genomic regions, and random sequences. We found sequence-encoded regions of unusually low bendability within nucleosome-depleted regions upstream of transcription start sites (TSSs). Low bendability of linker DNA inhibits nucleosome sliding into the linker by the chromatin remodeller INO80, which explains how INO80 can define nucleosome-depleted regions in the absence of other factors2. Chromosome-wide, nucleosomes were characterized by high DNA bendability near dyads and low bendability near linkers. This contrast increases for deeper gene-body nucleosomes but disappears after random substitution of synonymous codons, which suggests that the evolution of codon choice has been influenced by DNA mechanics around gene-body nucleosomes. Furthermore, we show that local DNA mechanics affect transcription through TSS-proximal nucleosomes. Overall, this genome-scale map of DNA mechanics indicates a 'mechanical code' with broad functional implications.
Zhang L, Zuris JA, Viswanathan R, Edelstein JN, Turk R, Thommandru B, Rube T, Glenn SE, Collingwood MA, Bode NM, et al. AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines. Nat. Commun. 2021;12(1):4500.

Publisher's Version

Though AsCas12a fills a crucial gap in the current genome editing toolbox, it exhibits relatively poor editing efficiency, restricting its overall utility. Here we isolate an engineered variant, “AsCas12a Ultra”, that increased editing efficiency to nearly 100% at all sites examined in HSPCs, iPSCs, T cells, and NK cells. We show that AsCas12a Ultra maintains high on-target specificity thereby mitigating the risk for off-target editing and making it ideal for complex therapeutic genome editing applications. We achieved simultaneous targeting of three clinically relevant genes in T cells at >90% efficiency and demonstrated transgene knock-in efficiencies of up to 60%. We demonstrate site-specific knock-in of a CAR in NK cells, which afforded enhanced anti-tumor NK cell recognition, potentially enabling the next generation of allogeneic cell-based therapies in oncology. AsCas12a Ultra is an advanced CRISPR nuclease with significant advantages in basic research and in the production of gene edited cell medicines.
Bussemaker HH, Rastogi C, Rube HT. Systems and methods for analyzing sequencing data. 2021.
Zhang L, Rube T, Vakulskas CA, Behlke MA, Bussemaker HJ, Pufall MA. Systematic in vitro profiling of off-target affinity, cleavage and efficiency for CRISPR enzymes. Nucleic Acids Res. 2020;48(9):5037–5053.

Publisher's Version

CRISPR RNA-guided endonucleases (RGEs) cut or direct activities to specific genomic loci, yet each has off-target activities that are often unpredictable. We developed a pair of simple in vitro assays to systematically measure the DNA-binding specificity (Spec-seq), catalytic activity specificity (SEAM-seq) and cleavage efficiency of RGEs. By separately quantifying binding and cleavage specificity, Spec/SEAM-seq provides detailed mechanistic insight into off-target activity. Feature-based models generated from Spec/SEAM-seq data for SpCas9 were consistent with previous reports of its in vitro and in vivo specificity, validating the approach. Spec/SEAM-seq is also useful for profiling less-well characterized RGEs. Application to an engineered SpCas9, HiFi-SpCas9, indicated that its enhanced target discrimination can be attributed to cleavage rather than binding specificity. The ortholog ScCas9, on the other hand, derives specificity from binding to an extended PAM. The decreased off-target activity of AsCas12a (Cpf1) appears to be primarily driven by DNA-binding specificity. Finally, we performed the first characterization of CasX specificity, revealing an all-or-nothing mechanism where mismatches can be bound, but not cleaved. Together, these applications establish Spec/SEAM-seq as an accessible method to rapidly and reliably evaluate the specificity of RGEs, Cas::gRNA pairs, and gain insight into the mechanism and thermodynamics of target discrimination.
Kribelbauer JF, Loker RE, Feng S, Rastogi C, Abe N, Rube T, Bussemaker HJ, Mann RS. Context-dependent gene regulation by homeodomain transcription factor complexes revealed by shape-readout deficient proteins. Mol. Cell. 2020;78(1):152–167.e11.

Publisher's Version

Eukaryotic transcription factors (TFs) form complexes with various partner proteins to recognize their genomic target sites. Yet, how the DNA sequence determines which TF complex forms at any given site is poorly understood. Here, we demonstrate that high-throughput in vitro DNA binding assays coupled with unbiased computational analysis provide unprecedented insight into how different DNA sequences select distinct compositions and configurations of homeodomain TF complexes. Using inferred knowledge about minor groove width readout, we design targeted protein mutations that destabilize homeodomain binding both in vitro and in vivo in a complex-specific manner. By performing parallel systematic evolution of ligands by exponential enrichment sequencing (SELEX-seq), chromatin immunoprecipitation sequencing (ChIP-seq), RNA sequencing (RNA-seq), and Hi-C assays, we not only classify the majority of in vivo binding events in terms of complex composition but also infer complex-specific functions by perturbing the gene regulatory network controlled by a single complex.
Rastogi C, Rube T, Kribelbauer JF, Crocker J, Loker RE, Martini GD, Laptenko O, Freed-Pastor WA, Prives C, Stern DL, et al. Accurate and sensitive quantification of protein-DNA binding affinity. Proc. Natl. Acad. Sci. U. S. A. 2018;115(16):E3692–E3701.

Publisher's Version

Transcription factors (TFs) control gene expression by binding to genomic DNA in a sequence-specific manner. Mutations in TF binding sites are increasingly found to be associated with human disease, yet we currently lack robust methods to predict these sites. Here, we developed a versatile maximum likelihood framework named No Read Left Behind (NRLB) that infers a biophysical model of protein-DNA recognition across the full affinity range from a library of in vitro selected DNA binding sites. NRLB predicts human Max homodimer binding in near-perfect agreement with existing low-throughput measurements. It can capture the specificity of the p53 tetramer and distinguish multiple binding modes within a single sample. Additionally, we confirm that newly identified low-affinity enhancer binding sites are functional in vivo, and that their contribution to gene expression matches their predicted affinity. Our results establish a powerful paradigm for identifying protein binding sites and interpreting gene regulatory sequences in eukaryotic genomes.

Rube Lab

Mathematical Biology | Genomics | Biophysics

Publications

2011

2009